ABSTRACT
Objective: this study was performed to analyze the morphological changes in primary oocyte and zona pellucida of primary follicle in prepubertal albino rats of three weeks ago after exposure to gamma radiation
Material and Methods: 72 female albino rats of age of three weeks were used. Out of these 24, received 4.5 Gray, another 24 received 8.3 Gray of Gamma radiation, and the rest of them received sham radiation. Ovaries of rats were dissected after one day, 7 days and 14 days after irradiation. Five random histological sections of each ovary were examined
Results: intense necrosis was evident in oocyte and granulosa cells of primary follicles after one day- post radiation. The zona pellucid a remained in contact with the oocyte and granulosa cells in initial phase of atresia. With progressing atresia, zona pellucida lost its contact with the oocytes and granulosa cells. Disintegration of zona pellucida started after development of apoptotic bodies in primary follicles. Disintegrated zona pellucida disappeared rapidly in experimental groups, particularly in high dose group. The basement membrane was irregular and disrupted in atretic follicles
Conclusion: therefore, we can conclude that gamma radiation induces rapid necrosis and cyst formation in primary oocyte. Disintegration of zona pellucida with progression of atresia in ovarian follicle was evident. Gamma radiation also produces irregular, disrupted and thinner basement membrane
ABSTRACT
Purpose: to analyze the morphometric changes, including diameter of ovary and oocytes in ovarian follicles as well as histological changes in these follicles in prepubertal rats after exposure to gamma radiation
Materials and Methods: 108 albino rats of 3wks age were the subjects of this study. Out of them, randomly chosen 36 animals received 4.5Gy and next 36 received 8.3Gy of gamma radiation, while rest of them received sham radiation. Ovaries of animals were dissected out 2hrs, 4hrs, 6hrs, one day, 7days and 14days after irradiation. Micrometry was performed for diameter of ovaries and oocytes in ovarian follicles. Histological examination of five random sections of each ovary was performed
Results: there was a dose related reduction in mean diameter of ovary and diameter of oocyte in primordial and primary follicles within 7 days. Oocytes in primordial and primary follicles also showed necrotic changes within 7days. Granulosa cells in primary follicles showed intense apoptosis during first six hours, followed by necrosis and development of cystic cavities containing remnants of oocyte and granulosa cells. Apoptosis, necrosis and cyst formation were not observed in granulosa cells of primordial follicles
Conclusion: therefore, it is concluded that gamma ionizing radiation induces reduction in diameter of ovaries and oocytes of primordial and primary follicles. It causes necrosis of oocytes and sequential changes including intense apoptosis and necrotic degeneration of granulosa cells in primary follicles leading to formation of abundant follicular cysts. It induces intense reduction in ovarian follicular reserve